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Journal / Biochemistry / PCR Mutagenesis, Kloning, Expression, Fast…
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JoVE Journal Biochemistry
PCR Mutagenesis, Kloning, Expression, Fast Protein Rensning Protokoller og krystallisering af wild type og mutant former for Tryptophan Synthase
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PCR Mutagenesis, Kloning, Expression, Fast Protein Rensning Protokoller og krystallisering af wild type og mutant former for Tryptophan Synthase

Article DOI: 10.3791/61839-v • 09:31 min • September 26th, 2020
September 26th, 2020 •
1, 2, 1, 2
1Department of Chemistry, University of California-Riverside, 2Department of Biochemistry, University of California-Riverside

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Denne artikel præsenterer en række på hinanden følgende metoder til udtryk og rensning af Salmonella typhimurium tryptophan synthase comp denne protokol et hurtigt system til at rense protein kompleks på en dag. Overdækkede metoder er stedsstyret mutagenese, proteinudtryk i Escherichia coli, affinitetskromatografi, gelfiltreringskromatografi og krystallisering.

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Biokemi Tryptophan synthase rekombinant protein mutant protein proteinudtryk proteinrensning ammoniumsulfatudfældning størrelsesudelukkelseskromatografi proteinkrystallisering
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