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JoVE Journal Biochemistry
Co-translationale Insertion von Membranproteinen in vorgeformte Nanoscheiben
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Co-translationale Insertion von Membranproteinen in vorgeformte Nanoscheiben

Article DOI: 10.3791/61844-v
November 19th, 2020 •
1, 1, 1, 1
1Institute of Biophysical Chemistry, Goethe University Frankfurt

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November 19th, 2020

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Die kotranslationale Insertion in vorgeformte Nanoscheiben ermöglicht es, zellfrei synthetisierte Membranproteine in definierten Lipidumgebungen ohne Kontakt mit Detergenzien zu untersuchen. Dieses Protokoll beschreibt die Vorbereitung wesentlicher Systemkomponenten und die kritischen Parameter zur Verbesserung der Expressionseffizienz und Probenqualität.

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