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Engenharia de Genoma de Células B Humanas Primárias Usando CRISPR/Cas9
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Engenharia de Genoma de Células B Humanas Primárias Usando CRISPR/Cas9

Article DOI: 10.3791/61855-v • 08:20 min • November 3rd, 2020
November 3rd, 2020 •
1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3
1Department of Pediatrics, University of Minnesota, 2Center for Genomic Engineering, University of Minnesota, 3Masonic Cancer Center, University of Minnesota

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Aqui fornecemos um protocolo detalhado passo a passo para a engenharia do genoma baseada em CRISPR/Cas9 das células B humanas primárias para nocaute genético (KO) e knock-in (KI) para estudar funções biológicas de genes em células B e o desenvolvimento de terapêuticas de células B.

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Biologia Edição 165 CRISPR/Cas9 engenharia de genomas AAV recombinante edição de genes células B humanas primárias
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