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Preparation of Decellularized Kidney Scaffolds in Rats

Article DOI: 10.3791/61856-v • 06:09 min • March 18th, 2021
March 18th, 2021 •
1, 1, 2, 2, 3, 4, 5, 6
1Department of Internal Medicine and Biomedical Research Institute, Pusan National University Hospital, 2Biomedical Research Institute, Pusan National University Hospital, 3Department of Plastic Surgery, Pusan National University Hospital, 4Department of Otorhinolaryngology - Head and Neck Surgery, Yangsan Pusan National University Hospital, 5Department of Otorhinolaryngology - Head and Neck Surgery, Pusan National University Hospital, 6Department of Pathology, Pusan National University Hospital

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Summary
Automatic Translation

This protocol introduces a method to develop a scaffold using decellularized rat kidneys. The protocol includes decellularization and recellularization processes to confirm bioavailability. Decellularization is performed using Triton X-100 and sodium dodecyl sulfate.

Tags

Decellularized Kidney Scaffolds Rats Vascularized Scaffolds Rat Model Differentiated Stem Cells Decellularization Protocol ECM Ultrastructure Transplantation Medicine Tissue Engineering Sprague Dawley Rat Warming Pad Rectal Thermometer Probe Anesthetizing Supine Position Abdomen Germicidal Soap Betadine Solution Ethanol Solution Sterile Drape Abdominal Incision Left Kidney Ureter Abdominal Aorta Inferior Vena Cava Tissue Dissection Perfusion Solution Heparin PFA Solution
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