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Journal / Biochemistry / Laservrije hydroxyl radicale eiwitvoetafdruk om…
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Biochemistry

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JoVE Journal Biochemistry
Laservrije hydroxyl radicale eiwitvoetafdruk om structurele analyse van eiwitten in hogere orde uit te voeren
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Laservrije hydroxyl radicale eiwitvoetafdruk om structurele analyse van eiwitten in hogere orde uit te voeren

Article DOI: 10.3791/61861-v • 09:59 min • June 4th, 2021
June 4th, 2021 •
1, 1, 1,2,3, 2
1GenNext Technologies Inc., 2Department of Biomolecular Sciences, School of Pharmacy, University of Mississippi, 3Department of Chemistry and Biochemistry, University of Mississippi

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Dit protocol presenteert een methode om inline radicale dosimetrie en een plasma lichtbron te gebruiken om flash oxidatie eiwit footprinting uit te voeren. Deze methode vervangt de gevaarlijke UV-laser om de reproduceerbaarheid van snelle fotochemische oxidatie van eiwitstudies te vereenvoudigen en te verbeteren.

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Biochemie Biofarmaceutisch Biosimilar Hogere Orde Structuur Snelle Fotochemische Oxidatie van Eiwitten (FPOP) Hydroxyl Radical Protein Footprinting (HRPF) Epitoop Mapping Receptor-Drug Interaction Protein-Protein Interaction
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