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Journal / Biology / An Antegrade Perfusion Method for Cardiomyocyte…
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An Antegrade Perfusion Method for Cardiomyocyte Isolation from Mice
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An Antegrade Perfusion Method for Cardiomyocyte Isolation from Mice

Article DOI: 10.3791/61866-v • 08:33 min • May 19th, 2021
May 19th, 2021 •
1, 1, 1, 1
1Department of Physiology, Shiga University of Medical Science

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Summary
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We developed a simple method for isolating high quality individual mouse heart cells by the antegrade perfusion technique. This method is Langendorff-free and useful for isolating ventricular and atrial myocytes or interstitial cells, such as cardiac fibroblasts or progenitors.

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Antegrade Perfusion Method Cardiomyocyte Isolation Mice Langendorff-free Method Heart Cells Retrograde Perfusion LTA Cannulation Mouse Heart Harvest Plastic Transfer Pipette Heart Excision Ice Cold CIB-EGTA Culture Dish Heart Stand Stereoscopic Microscope Aorta Trimming
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