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Journal / Immunology and Infection / En DNA/Ki67-basert flowcytometrianalyse for…
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Immunology and Infection

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JoVE Journal Immunology and Infection
En DNA/Ki67-basert flowcytometrianalyse for cellesyklusanalyse av antigenspesifikke CD8 T-celler hos vaksinerte mus
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En DNA/Ki67-basert flowcytometrianalyse for cellesyklusanalyse av antigenspesifikke CD8 T-celler hos vaksinerte mus

Article DOI: 10.3791/61867-v • 09:17 min • January 5th, 2021
January 5th, 2021 •
*1,2, *1,2, 3, 4, 5, 5, 2,6, 1
1Institute of Molecular Biology and Pathology, National Research Council of Italy (CNR), 2Department of Molecular Medicine, University of Rome “Sapienza”, 3Center for Life Nano Science, Istituto Italiano di Tecnologia, 4Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 5Reithera Srl, 6IRCCS, Neuromed
* These authors contributed equally

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Clonal ekspansjon er et sentralt trekk ved antigenspesifikk T-cellerespons. Cellesyklusen til antigenresponserende T-celler har imidlertid blitt dårlig undersøkt, blant annet på grunn av tekniske begrensninger. Vi beskriver en strømningscytometrisk metode for å analysere clonally ekspanderende antigenspesifikke CD8 T-celler i milt og lymfeknuter av vaksinerte mus.

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Immunologi og infeksjon utgave 167 antigenspesifikke CD8 T-celler cellesyklus Ki67 DNA-fargestoff strømningscytometri milt lymfeknuter mus
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