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JoVE Journal Bioengineering
Fabricación de matrices de microtejidos cardíacos 3D utilizando cardiomiocitos humanos derivados de iPSC, fibroblastos cardíacos y células endoteliales
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Fabricación de matrices de microtejidos cardíacos 3D utilizando cardiomiocitos humanos derivados de iPSC, fibroblastos cardíacos y células endoteliales

Article DOI: 10.3791/61879-v • 10:37 min • March 14th, 2021
March 14th, 2021 •
1, 1, 1, 1,2,3
1Stanford Cardiovascular Institute, Stanford University School of Medicine, 2Department of Medicine, Division of Cardiovascular Medicine, Stanford University School of Medicine, 3Department of Radiology, Stanford University School of Medicine

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Aquí, describimos una metodología fácil de usar para generar matrices de microtejidos cardíacos autoensamblados en 3D compuestos por cardiomiocitos derivados de células madre pluripotentes prediferenciadas inducidas por humanos, fibroblastos cardíacos y células endoteliales. Esta técnica fácil de usar y de baja capacidad de células para generar microtejidos cardíacos se puede implementar para el modelado de enfermedades y las primeras etapas del desarrollo de fármacos.

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Bioingeniería Número 169 iPSC cardiomiocitos células endoteliales fibroblastos microtejidos autoensamblaje
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