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Immunology and Infection

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JoVE Journal Immunology and Infection
Labelvrije kwantitatieve proteomics workflow voor discovery-driven host-pathogen interacties
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Labelvrije kwantitatieve proteomics workflow voor discovery-driven host-pathogen interacties

Article DOI: 10.3791/61881-v • 05:37 min • October 20th, 2020
October 20th, 2020 •
1, 1, 1
1Molecular and Cellular Biology Department, University of Guelph

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Hier presenteren we een protocol om het samenspel tussen gastheer en ziekteverwekker te profileren tijdens infectie door op massaspectrometrie gebaseerde proteomics. Dit protocol maakt gebruik van labelvrije kwantificering om veranderingen in eiwitrijkdom van zowel gastheer (bijv. macrofagen) als ziekteverwekker (bijv. Cryptococcus neoformans)in één experiment te meten.

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Immunologie en infectie Op massaspectrometrie gebaseerde proteomics labelvrije kwantificering gastheer-pathogene interacties zoogdiercelcultuur schimmelpathogene Cryptococcus neoformans
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