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Pupal en volwassen injecties voor RNAi en CRISPR Gen editing in Nasonia vitripennis
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Pupal en volwassen injecties voor RNAi en CRISPR Gen editing in Nasonia vitripennis

Article DOI: 10.3791/61892-v • 08:41 min • December 4th, 2020
December 4th, 2020 •
*1, *1, 2, 1
1Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, 2Department of Entomology, The Center for Infectious Disease Dynamics, The Huck Institutes of the Life Sciences, The Pennsylvania State University
* These authors contributed equally

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Hier beschrijven we methoden voor efficiënte pop- en volwassen injecties in Nasonia vitripennis als toegankelijke alternatieven voor embryomicro-injectie, waardoor functionele analyse van genen van belang mogelijk is met behulp van RNA-silencing via RNA-interferentie (RNAi) of gen knock-out via CRISPR / Cas9 genoombewerking.

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Biologie Nasonia vitripennis CRISPR/Cas9 ReMOT BAPC-assisted CRISPR Adult injections RNAi
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