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Journal / Biochemistry / Rastgele Mikrotohum Matris Taraması Ile Protein…
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Biochemistry
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JoVE Journal Biochemistry
Rastgele Mikrotohum Matris Taraması Ile Protein Kristallerinin I3C ile Türevleştirilmesi
On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature
Journal (Biochemistry)
On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature
Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography
Journal (Biochemistry)
Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography
 
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Rastgele Mikrotohum Matris Taraması Ile Protein Kristallerinin I3C ile Türevleştirilmesi

Article DOI: 10.3791/61894-v • 14:04 min • January 16th, 2021
January 16th, 2021 •
*1, *1,2, 1,2, 1
1School of Biological Sciences, The University of Adelaide, 2Institute of Photonics and Advanced Sensing (IPAS), School of Biological Sciences, The University of Adelaide
* These authors contributed equally

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Bu makalede, seyrek matris ekranlarda yeni kristalleşme koşulları oluşturmak için mikrotohumlama kullanarak I3C (5-amino-2,4,6-triiodoishthalic asit) ile türemiş protein kristalleri oluşturmak için bir yöntem sunar. Tepsiler sıvı dağıtım robotları kullanılarak veya elle kurulabilir.

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Biyokimya Sayı 167 tohumlama rastgele mikrotohum matris taraması rMMS protein kristalografisi phasing Sihirli Üçgen I3C
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