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Journal / Biochemistry / De afleiding van eiwitkristallen met I3C met…
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Biochemistry
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JoVE Journal Biochemistry
De afleiding van eiwitkristallen met I3C met behulp van Random Microseed Matrix Screening
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De afleiding van eiwitkristallen met I3C met behulp van Random Microseed Matrix Screening

Article DOI: 10.3791/61894-v • 14:04 min • January 16th, 2021
January 16th, 2021 •
*1, *1,2, 1,2, 1
1School of Biological Sciences, The University of Adelaide, 2Institute of Photonics and Advanced Sensing (IPAS), School of Biological Sciences, The University of Adelaide
* These authors contributed equally

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Dit artikel presenteert een methode om eiwitkristallen te genereren die zijn afgeleid met I3C (5-amino-2,4,6-triiodoisoftaalzuur) met behulp van microseeding om nieuwe kristallisatieomstandigheden in schaarse matrixschermen te genereren. De trays kunnen worden opgezet met behulp van vloeibare doseerrobots of met de hand.

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Biochemie zaaien willekeurige microseed matrix screening rMMS eiwit kristallografie fasering de Magische Driehoek I3C
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