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Journal / Biochemistry / Derivazione di cristalli proteici con I3C…
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Biochemistry
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JoVE Journal Biochemistry
Derivazione di cristalli proteici con I3C utilizzando lo screening casuale della matrice microseed
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Derivazione di cristalli proteici con I3C utilizzando lo screening casuale della matrice microseed

Article DOI: 10.3791/61894-v • 14:04 min • January 16th, 2021
January 16th, 2021 •
*1, *1,2, 1,2, 1
1School of Biological Sciences, The University of Adelaide, 2Institute of Photonics and Advanced Sensing (IPAS), School of Biological Sciences, The University of Adelaide
* These authors contributed equally

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Questo articolo presenta un metodo per generare cristalli proteici derivati con I3C (acido 5-ammino-2,4,6-triiodoisoftalico) utilizzando il microseeding per generare nuove condizioni di cristallizzazione negli schermi a matrice sparsa. I vassoi possono essere impostati utilizzando robot di erogazione di liquidi o a mano.

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Biochimica Numero 167 semina screening casuale della matrice microseed rMMS cristallografia proteica phasing il Triangolo Magico I3C
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