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Journal / Biochemistry / Derivatização de Cristais de Proteína com I3C…
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Biochemistry
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JoVE Journal Biochemistry
Derivatização de Cristais de Proteína com I3C usando Triagem de Matriz Microseada Aleatória
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Derivatização de Cristais de Proteína com I3C usando Triagem de Matriz Microseada Aleatória

Article DOI: 10.3791/61894-v • 14:04 min • January 16th, 2021
January 16th, 2021 •
*1, *1,2, 1,2, 1
1School of Biological Sciences, The University of Adelaide, 2Institute of Photonics and Advanced Sensing (IPAS), School of Biological Sciences, The University of Adelaide
* These authors contributed equally

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Este artigo apresenta um método para gerar cristais proteicos derivados com I3C (5-amino-2,4,6-triiodoisophthalic ácido) utilizando microseeding para gerar novas condições de cristalização em telas de matriz esparsas. As bandejas podem ser configuradas usando robôs de distribuição de líquidos ou à mão.

Tags

Bioquímica Edição 167 semeadura triagem aleatória de matriz de microseed rMMS cristalografia proteica eliminação triângulo mágico I3C
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