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Journal / Immunology and Infection / Mikrofluidische Co-Kulturmodelle zur Analyse der…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Mikrofluidische Co-Kulturmodelle zur Analyse der Immunantwort in in vitro Tumormikroumgebungen
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Mikrofluidische Co-Kulturmodelle zur Analyse der Immunantwort in in vitro Tumormikroumgebungen

Article DOI: 10.3791/61895-v • 07:46 min • April 30th, 2021
April 30th, 2021 •
1, 1, 1, 2, 3, 3, 2, 3,4, 1
1CNR Institute for Photonics and Nanotechnology, 2Dept. of Oncology and Molecular Medicine, Istituto Superiore di Sanità, 3Istituto di Patologia Generale, Università Cattolica del Sacro Cuore, 4Tumor Immunology and Immunotherapy Unit, IRCCS Regina Elena National Cancer Institute

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Im Zeitalter der Immuntherapie und des genomischen Profilings von Einzelzellen erfordert die Krebsbiologie neuartige In-vitro- und Computerwerkzeuge, um die Tumor-Immun-Schnittstelle in einem geeigneten raumzeitlichen Kontext zu untersuchen. Wir beschreiben Protokolle zur Nutzung von mikrofluidischen Kokulturen des Tumor-Immunsystems in 2D- und 3D-Umgebungen, die mit einer dynamischen, multiparametrischen Überwachung zellulärer Funktionen kompatibel sind.

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Diesen Monat in JoVE Ausgabe 170 Organs-on-Chip Tumorimmunologie Krebs-Immun-Crosstalk Tumormikroumgebung Immunkontexture zelluläre Dynamik und Interaktionen Mikrofluidik immunkompetenter Tumor-on-Chip Immunonkologie Zellverfolgung
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