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Cerebellar regional disseksjon for molekylær analyse
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Cerebellar regional disseksjon for molekylær analyse

Article DOI: 10.3791/61922-v • 08:51 min • December 5th, 2020
December 5th, 2020 •
1, 1
1Department of Neuroscience, University of Minnesota

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Ulike cerebellar regioner har blitt implisert for å spille en rolle i distinkte atferdsmessige utganger, men de underliggende molekylære mekanismene forblir ukjente. Dette arbeidet beskriver en metode for å reprodusere og raskt dissekere cerebellar cortex av halvkule, fremre og bakre regioner i vermis, og de dype cerebellar kjernene for å sonde for molekylære forskjeller ved å isolere RNA og teste for forskjeller i genuttrykk.

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Nevrovitenskap Utgave 166 Cerebellum Regionalisering Dype Cerebellar Nuclei Cerebellar Cortex RNA RTqPCR
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