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Genetics

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JoVE Journal Genetics
Sandfly (Phlebotomus papatasi) Embryo Mikroinjeksjon for CRISPR/Cas9 Mutagenese
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Sandfly (Phlebotomus papatasi) Embryo Mikroinjeksjon for CRISPR/Cas9 Mutagenese

Article DOI: 10.3791/61924-v • 05:44 min • November 17th, 2020
November 17th, 2020 •
1, 1, 1, 1, 2, 2
1Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 2University of Maryland Insect Transformation Facility, The Institute for Bioscience and Biotechnology Research

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Denne protokollen beskriver trinnene i CRISPR/Cas9 målrettet mutagenese i sandfluer: embryosamling, injeksjon, insektoppdrett og identifisering samt utvalg av mutasjoner av interesse.

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Denne måneden i JoVE utgave 165 insekt genomredigering embryoinjeksjon
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