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Journal / Bioengineering / 使用DNA对细胞进行简单、经济、模块化的图案化…
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Bioengineering

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JoVE Journal Bioengineering
使用DNA对细胞进行简单、经济、模块化的图案化
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使用DNA对细胞进行简单、经济、模块化的图案化

Article DOI: 10.3791/61937-v • 08:59 min • February 24th, 2021
February 24th, 2021 •
1, 2, 1, 3, 4,5,6, 7,8, 9, 2,6,10
1Graduate Program in Bioengineering, University of California San Francisco and University of California Berkeley, 2Department of Pharmaceutical Chemistry, University of California San Francisco, 3Scribe Biosciences, 4Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, 5California Institute for Quantitative Biosciences, University of California San Francisco, 6Chan Zuckerberg Biohub, University of California San Francisco, 7Department of Chemical & Biomolecular Engineering, University of California Berkeley, 8Helen Wills Neuroscience Institute, University of California Berkeley, 9Department of Mechanical Engineering, University of California Berkeley, 10Center for Cellular Construction, University of California San Francisco

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在这里，我们提出了一种使用DNA程序化粘附的单细胞分辨率的微模式细胞方案。该协议使用台式光刻平台在载玻片上创建DNA寡核苷酸的图案，然后用市售的互补寡核苷酸标记细胞膜。寡核苷酸的杂交导致程序化的细胞粘附。

Tags

生物工程，第168期，微图案化，组织工程，生物工程，3D细胞培养，光刻，微加工，细胞生物学
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