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Mikrodissektion af musehjerne i funktionelt og anatomisk forskellige regioner
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Mikrodissektion af musehjerne i funktionelt og anatomisk forskellige regioner

Article DOI: 10.3791/61941-v • 08:06 min • February 15th, 2021
February 15th, 2021 •
1, 1, 2, 3, 3, 2, 4, 2
1The Geneva Foundation, Medical Readiness Systems Biology, Walter Reed Army Institute of Research, 2Walter Reed Army Institute of Research, Medical Readiness Systems Biology, Center for Military Psychiatry and Neuroscience, 3ORISE Fellow, Medical Readiness Systems Biology, Walter Reed Army Institute of Research, 4Headquarter, Walter Reed Army Institute of Research

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Vi præsenterer en praktisk, trin-for-trin, hurtig protokol til fjernelse af musehjerne og dissektion af diskrete områder fra frisk hjernevæv. At opnå hjerneområder til molekylær analyse er blevet rutine i mange neurovidenskabslaboratorier. Disse hjerneområder fryses straks for at opnå transkriptomiske data af høj kvalitet til analyse på systemniveau.

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Neurovidenskab Udgave 168 Hjernens anatomi mus hjerne dissektion hjerneatlas molekylære assays
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