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Induction and Analysis of Oxidative Stress in Sleeping Beauty Transposon-Transfected Human Retinal Pigment Epithelial Cells

Article DOI: 10.3791/61957-v • 10:00 min • December 11th, 2020
December 11th, 2020 •
1,2, 1,2, 1,2, 1, 3, 1,2
1Experimental Ophthalmology, University of Geneva, 2Department of Ophthalmology, University Hospitals of Geneva, 3Max Delbrück Center for Molecular Medicine

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Summary
Automatic Translation

We present a protocol for the development and use ofan oxidative stress-model by treating retinal pigment epithelial cells with H2O2, analyzing cell morphology, viability, density, glutathione, and UCP-2 level. It is a useful model to investigate the antioxidant effect of proteins secreted by transposon-transfected cells to treat neuroretinal degeneration.

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Induction Analysis Oxidative Stress Sleeping Beauty Transposon Human Retinal Pigment Epithelial Cells Antioxidant Effects Age-related Macular Degeneration Neurodegenerative Diseases Hydrogen Peroxide Model Oxidant Agent Single Pulse Delivery Cell Counting Thais Bascuas Gabriele Thumann's Laboratory Conditioned Medium Preparation Transfection PEDF GM-CSF ARPE-19 Cell Line T-75 Flasks Complete Medium Concentration Supernatant Replacement Protein Purification Histidine-tagged GM-CSF And PEDF Proteins
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