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Journal / Neuroscience / Forberedelse af akutte skiver fra Dorsal…
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Neuroscience
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JoVE Journal Neuroscience
Forberedelse af akutte skiver fra Dorsal Hippocampus til helcelleoptagelse og neuronal rekonstruktion i dentate gyrus af voksne mus
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Forberedelse af akutte skiver fra Dorsal Hippocampus til helcelleoptagelse og neuronal rekonstruktion i dentate gyrus af voksne mus

Article DOI: 10.3791/61980-v • 10:45 min • April 3rd, 2021
April 3rd, 2021 •
1, 1, 1
1Institute of Physiology, Christian-Albrechts-University Kiel

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Vi præsenterer en protokol til forberedelse af akutte skiver fra musens dorsale mellemliggende hippocampus. Vi sammenligner dette tværgående præparat med korona udskæring med hensyn til kvaliteten af optagelser og bevarelse af morfologiske træk ved registrerede neuroner.

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Neurovidenskab Dorsal hippocampus dentate gyrus parvalbumin granulatcelle interneuron patch-clamp optagelse tværgående
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