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Elektroporationsbaserad genetisk modifiering av primära mänskliga pigmentepitelceller med hjälp av Törnrosa Transposon System
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Elektroporationsbaserad genetisk modifiering av primära mänskliga pigmentepitelceller med hjälp av Törnrosa Transposon System

Article DOI: 10.3791/61987-v • 07:04 min • February 4th, 2021
February 4th, 2021 •
1, 2,3, 4,5, 4, 6, 7, 1, 2,3
1Department of Ophthalmology, University Hospital RWTH Aachen, 2Experimental Ophthalmology, University of Geneva, 3Department of Ophthalmology, University Hospitals of Geneva, 4Université de Paris, CNRS, INSERM, UTCBS, Unité des technologies Chimiques et Biologiques pour la Santé, 5Chimie ParisTech, PSL Research University, 6Max Delbrück Center for Molecular Medicine in the Helmholtz Association, 7Division of Medical Biotechnology, Paul-Ehrlich-Institute

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Vi har utvecklat ett protokoll för att transfektera primära humana pigmentepitelceller genom elektroporering med genen som kodar för pigmentepitel-härledd faktor (PEDF) med hjälp av Törnrosa (SB) transposonsystem. Framgångsrik transfektion demonstrerades genom kvantitativ polymeraskedjereaktion (qPCR), immunoblotting och enzymbunden immunosorbentanalys (ELISA).

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Medicin utgåva 168 åldersrelaterad makuladegeneration AMD kapillärelektroporationssystem icke-viral transfektion pigmentepitel-härledd faktor PEDF primära pigmentepitelceller Törnrosa transposonsystem SB
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