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JoVE Journal Genetics
Direkte omprogrammering af humane fibroblaster til myoblaster for at undersøge behandlingsformer for neuromuskulære lidelser
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Direkte omprogrammering af humane fibroblaster til myoblaster for at undersøge behandlingsformer for neuromuskulære lidelser

Article DOI: 10.3791/61991-v • 10:28 min • April 3rd, 2021
April 3rd, 2021 •
1, 1, 1, 2, 2,3,4,6, 5, 1,6, 1,6
1Center for Gene Therapy, The Research Institute at Nationwide Children’s Hospital, 2Center for Cardiovascular Research, The Research Institute at Nationwide Children’s Hospital, 3The Heart Center, Nationwide Children’s Hospital, 4Division of Genetic and Genomic Medicine, Nationwide Children’s Hospital, 5Department of Human Genetics, The University of Utah School of Medicine, 6Department of Pediatrics, The Ohio State University

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Denne protokol beskriver konverteringen af hudfibroblaster til myoblaster og deres differentiering til myorør. Cellelinjerne er afledt af patienter med neuromuskulære lidelser og kan bruges til at undersøge patologiske mekanismer og til at teste terapeutiske strategier.

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Genetik Problem 170 Omprogrammering hTERT MyoD fibroblast myoblast neuromuskulær lidelse terapitest
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