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Journal / Genetics / Reprogramação Direta de Fibroblastos Humanos em…
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Genetics
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JoVE Journal Genetics
Reprogramação Direta de Fibroblastos Humanos em Myoblasts para investigar terapias para distúrbios neuromusculares
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Reprogramação Direta de Fibroblastos Humanos em Myoblasts para investigar terapias para distúrbios neuromusculares

Article DOI: 10.3791/61991-v • 10:28 min • April 3rd, 2021
April 3rd, 2021 •
1, 1, 1, 2, 2,3,4,6, 5, 1,6, 1,6
1Center for Gene Therapy, The Research Institute at Nationwide Children’s Hospital, 2Center for Cardiovascular Research, The Research Institute at Nationwide Children’s Hospital, 3The Heart Center, Nationwide Children’s Hospital, 4Division of Genetic and Genomic Medicine, Nationwide Children’s Hospital, 5Department of Human Genetics, The University of Utah School of Medicine, 6Department of Pediatrics, The Ohio State University

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Este protocolo descreve a conversão de fibroblastos de pele em míobios e sua diferenciação em miotubes. As linhas celulares são derivadas de pacientes com distúrbios neuromusculares e podem ser usadas para investigar mecanismos patológicos e testar estratégias terapêuticas.

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Genética Edição 170 Reprogramação hTERT MyoD fibroblasto míoblast distúrbio neuromuscular teste terapêutico
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