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JoVE Journal Bioengineering
Hurtig samling af multigenkonstruktioner ved hjælp af modulær golden gate-kloning
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Hurtig samling af multigenkonstruktioner ved hjælp af modulær golden gate-kloning

Article DOI: 10.3791/61993-v • 08:31 min • February 5th, 2021
February 5th, 2021 •
*1, *1, 1
1Department of Biological Sciences, University at Buffalo, State University of New York
* These authors contributed equally

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Målet med denne protokol er at give en detaljeret, trin-for-trin guide til samling af multi-gen konstruktioner ved hjælp af modulopbygget kloning system baseret på Golden Gate kloning. Den giver også anbefalinger til kritiske skridt for at sikre optimal montage baseret på vores erfaringer.

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