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Journal / Biochemistry / Utilización de Grafix para la detección de…
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JoVE Journal Biochemistry
Utilización de Grafix para la detección de interactores transitorios de subcomplejos de Saccharomyces cerevisiae Spliceosome
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Utilización de Grafix para la detección de interactores transitorios de subcomplejos de Saccharomyces cerevisiae Spliceosome

Article DOI: 10.3791/61994-v • 05:44 min • November 9th, 2020
November 9th, 2020 •
1, 1, 1, 1, 1
1Department of Biochemistry, Institute of Chemistry, University of Sao Paulo

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Aquí, describimos la utilización de Grafix (Gradient Fixation), una centrifugación de gradiente de glicerol en presencia de un ticulador, para identificar interacciones entre factores de empalme que se unen transitoriamente al complejo de espliceosomas.

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Bioquímica Número 165 subcomplejos de espliceosomas de levadura Grafix separación de complejos RNP empalme Saccharomyces cerevisiae,centrifugación por gradiente de glicerol
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