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Journal / Neuroscience / Modellazione in vitro per malattie neurologiche…
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Neuroscience
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JoVE Journal Neuroscience
Modellazione in vitro per malattie neurologiche utilizzando la conversione diretta dai fibroblasti alle cellule progenitrici neuronali e la differenziazione in astrociti
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Modellazione in vitro per malattie neurologiche utilizzando la conversione diretta dai fibroblasti alle cellule progenitrici neuronali e la differenziazione in astrociti

Article DOI: 10.3791/62016-v • 11:42 min • June 10th, 2021
June 10th, 2021 •
1, 1, 1, 1, 1, 1, 1,2
1Center for Gene Therapy, The Research Institute at Nationwide Children’s Hospital, 2College of Medicine, The Ohio State University

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Descriviamo un protocollo per riprogrammare i fibroblasti derivati dalla pelle umana in cellule progenitrici neuronali indotte (INPC) e la loro successiva differenziazione in Astrociti indotti (iA). Questo metodo porta a una generazione rapida e riproducibile di iNPC e iA in grandi quantità.

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Neuroscienze Numero 172 Modellazione in vitro riprogrammazione diretta conversione fibroblasti cellule progenitrici neuronali astrociti indotti
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