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Journal / Biology / Fluorescens in situ-hybridisering på…
JoVE Journal
Biology

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JoVE Journal Biology
Fluorescens in situ-hybridisering på DNA-halopreparat för att avslöja hela kromosomer, telomerer och genlokus
Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization (FISH)
Journal (Biology)
Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization (FISH)
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Fluorescens in situ-hybridisering på DNA-halopreparat för att avslöja hela kromosomer, telomerer och genlokus

Article DOI: 10.3791/62017-v • 09:07 min • March 4th, 2021
March 4th, 2021 •
1, 2, 1, 2
1Laboratory of Nuclear and Genomic Health, Centre for Genome Engineering and Maintenance, Division of Biosciences, Department of Life Sciences, College of Health, Medicine and Life Sciences, Brunel University London, 2Biosciences, Department of Clinical, Pharmaceutical and Biological Science, School of Life and Medical Sciences, University of Hertfordshire

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Genom att kombinera DNA-halopreparat med fluorescens in situ-hybridisering möjliggörs högupplöst analys av genomiska interaktioner med nukleoskelettet. Bifogat genom leder till hybridiserade fluorescerande signaler belägna inom de kvarvarande extraherade kärnorna, medan icke-bundet genom ligger i DNA-halo som omger restkärnorna.

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Denna månad i JoVE nummer 169 DNA-haloberedning kärnmatris nukleoskelett genomorganisation DNA-slingor kromosomterritorier utökade kromatinfibrer matrisfästregioner telomerer
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