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Kemisk bøjning af et renset DEC-205-rettet antistof med protein i fuld længde til målretning af musededritiske celler In Vitro og In Vivo
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Kemisk bøjning af et renset DEC-205-rettet antistof med protein i fuld længde til målretning af musededritiske celler In Vitro og In Vivo

Article DOI: 10.3791/62018-v • 10:35 min • February 5th, 2021
February 5th, 2021 •
1,2, 1,2,3, 1, 1,2, 4, 4, *1,2,5, *1,2
1Immune Regulation Group, Helmholtz Centre for Infection Research, 2Infection Immunology Group, Institute of Medical Microbiology, Infection Prevention and Control, Health Campus Immunology, Infectiology and Inflammation, Otto-von-Guericke University Magdeburg, 3Institute for Molecular and Clinical Immunology, Health Campus Immunology, Infectiology and Inflammation, Otto-von-Guericke University Magdeburg, 4Cellular Proteome Research, Helmholtz Centre for Infection Research, 5Experimental Pneumology, University Hospital of Pneumology, Health Campus Immunology, Infectiology and Inflammation, Otto-von-Guericke University Magdeburg
* These authors contributed equally

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Vi beskriver en protokol for den kemiske bøjning af modellen antigen ovalbumin til en endokytose receptor-specifikke antistof for in vivo dendritiske celle målretning. Protokollen omfatter rensning af antistoffet, kemisk bøjning af antigen, samt rensning af konjugaten og verifikation af effektiv bøjning.

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Kræftforskning Udgave 168 dendritisk cellemålretning dendritiske celler DEC-205 antigenlevering antigenkonjugation kemisk bøjning antistofmedieret endokytose vaccination antitumorimmunitet
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