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Kemisk konjugation av en renad DEC-205-riktad antikropp med fulllängdsprotein för inriktning på mus dendritiska celler in vitro och in vivo
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Kemisk konjugation av en renad DEC-205-riktad antikropp med fulllängdsprotein för inriktning på mus dendritiska celler in vitro och in vivo

Article DOI: 10.3791/62018-v • 10:35 min • February 5th, 2021
February 5th, 2021 •
1,2, 1,2,3, 1, 1,2, 4, 4, *1,2,5, *1,2
1Immune Regulation Group, Helmholtz Centre for Infection Research, 2Infection Immunology Group, Institute of Medical Microbiology, Infection Prevention and Control, Health Campus Immunology, Infectiology and Inflammation, Otto-von-Guericke University Magdeburg, 3Institute for Molecular and Clinical Immunology, Health Campus Immunology, Infectiology and Inflammation, Otto-von-Guericke University Magdeburg, 4Cellular Proteome Research, Helmholtz Centre for Infection Research, 5Experimental Pneumology, University Hospital of Pneumology, Health Campus Immunology, Infectiology and Inflammation, Otto-von-Guericke University Magdeburg
* These authors contributed equally

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Vi beskriver ett protokoll för kemisk konjugation av modellen antigen ovalbumin till en endocytosis receptor-specifika antikropp för in vivo dendritic cell inriktning. Protokollet omfattar rening av antikroppen, kemisk konjugation av antigenet samt rening av konjugat och verifiering av effektiv konjugation.

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Cancerforskning nummer 168 dendritisk cellinriktning dendritiska celler DEC-205 antigenleverans antigenkonjugation kemisk konjugation antikroppsmedierad endocytos vaccination antitumörimmunitet
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