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Refined CLARITY-Based Tissue Clearing for Three-Dimensional Fibroblast Organization in Healthy and Injured Mouse Hearts

Article DOI: 10.3791/62023-v • 07:10 min • May 16th, 2021
May 16th, 2021 •
1,2, 3, 2, 2
1Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, 2Division of Molecular Cardiovascular Biology, Cincinnati Children's Hospital Medical Center, 3Confocal Imaging Core, Cincinnati Children's Hospital Medical Center

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Summary
Automatic Translation

A refined method of tissue clearing was developed and applied to the adult mouse heart. This method was designed to clear dense, autofluorescent cardiac tissue, while maintaining labeled fibroblast fluorescence attributed to a genetic reporter strategy.

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Tissue Clearing CLARITY-based Tissue Clearing Fibroblast Organization Healthy Mouse Hearts Injured Mouse Hearts In Vivo Cell Types Injury Conditions Protocol Fluorescence Maintenance Background Tissue Autofluorescence Cell Staining Ethanol Soaked Gauze Pad Transverse Incision Xiphoid Process Deglove The Mouse Subcutaneous Abdominal Wall Tissue Rib Cage Superior Vena Cava Aorta Cold PBS 4%PFA Fixation Process Excise The Heart
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