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Live-cell Imaging af single-cell arrays (LISCA) - en alsidig teknik til at kvantificere Cellular Kinetics
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Live-cell Imaging af single-cell arrays (LISCA) - en alsidig teknik til at kvantificere Cellular Kinetics

Article DOI: 10.3791/62025-v • 10:24 min • March 18th, 2021
March 18th, 2021 •
1, 1, 1, 1
1Faculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-Universität München, Geschwister-Scholl-Platz 1, 80539 München, Germany

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Vi præsenterer en metode til erhvervelse af fluorescens reporter tid kurser fra enkelt celler ved hjælp af mikropatterned arrays. Protokollen beskriver forberedelsen af enkeltcellede arrays, opsætningen og driften af live-celle scanning time-lapse mikroskopi og en open source billedanalyse værktøj til automatiseret forvalg, visuel kontrol og sporing af celle-integreret fluorescens tid kurser pr vedhæftning site.

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Biologi Problem 169 Enkeltcelleanalyse mikrostrukturerede arrays time-lapse mikroskopi cellulær kinetik
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