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单细胞阵列的活细胞成像 (LISCA) - 量化细胞动力学的多功能技术
 
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单细胞阵列的活细胞成像 (LISCA) - 量化细胞动力学的多功能技术

Article DOI: 10.3791/62025
March 18th, 2021

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Summary March 18th, 2021

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我们提出了一种利用微模式阵列从单个细胞获取荧光记者时间课程的方法。该协议描述了单细胞阵列的准备、活细胞扫描延时显微镜的设置和操作,以及用于自动预选、可视控制和跟踪每个粘附站点的细胞集成荧光时间课程的开源图像分析工具。

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