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Live-cell Imaging af single-cell arrays (LISCA) - en alsidig teknik til at kvantificere Cellular Kinetics
 
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Live-cell Imaging af single-cell arrays (LISCA) - en alsidig teknik til at kvantificere Cellular Kinetics

Article DOI: 10.3791/62025
March 18th, 2021

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Summary March 18th, 2021

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Vi præsenterer en metode til erhvervelse af fluorescens reporter tid kurser fra enkelt celler ved hjælp af mikropatterned arrays. Protokollen beskriver forberedelsen af enkeltcellede arrays, opsætningen og driften af live-celle scanning time-lapse mikroskopi og en open source billedanalyse værktøj til automatiseret forvalg, visuel kontrol og sporing af celle-integreret fluorescens tid kurser pr vedhæftning site.

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