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Journal / Biology / Isolating and Imaging Live, Intact Pacemaker…
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Biology

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Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning
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Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning

Article DOI: 10.3791/62040-v • 07:38 min • April 30th, 2021
April 30th, 2021 •
1,2, 1, 1,3
1Department of Physiology & Cell Biology, University of Nevada, Reno School of Medicine, 2Department of Physiology & Membrane Biology, University of California School of Medicine, 3Department of Life & Health Sciences, Dundalk Institute of Technology

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Summary
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The goal of this protocol is to isolate intact pacemaker regions of the mouse renal pelvis using vibratome sectioning. These sections can then be used for in situ Ca2+ imaging to elucidate Ca2+ transient properties of pacemaker cells and other interstitial cells in vibratome slices.

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Isolating Imaging Live Intact Pacemaker Regions Mouse Renal Pelvis Vibratome Sectioning Smooth Muscle Organ Kidney Urine Ureter Bladder In Situ Environments Physiological Preparation Studying Renal Pelvis Pacemaking Technique Uniform Sections Vibratome Stage Cutting Process Internal Tissue Forceps Internal Dissection Scissors Intestines Abdominal Wall Proximal Duodenum Distal Colon Retroperitoneal Space Kidneys Ureter Tissue Forceps Dissection Scissors Connective Tissue Silicon Elastomer-coated Dish KRB Solution Adipose Tissue
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