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Journal / Immunology and Infection / Onderzoek naar aortaklepverkalking via isolatie…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Onderzoek naar aortaklepverkalking via isolatie en kweek van T-lymfocyten met behulp van feedercellen van bestraalde buffy coat
Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry
Journal (Immunology and Infection)
Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry
Author Spotlight: Development of a Method for Identifying Small Molecular Antagonists of β2 Integrin Activation (Video) | JoVE
Journal (Immunology and Infection)
Author Spotlight: Development of a Method for Identifying Small Molecular Antagonists of β2 Integrin Activation (Video) | JoVE
 
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Onderzoek naar aortaklepverkalking via isolatie en kweek van T-lymfocyten met behulp van feedercellen van bestraalde buffy coat

Article DOI: 10.3791/62059-v • 04:30 min • February 4th, 2021
February 4th, 2021 •
*1,2, *1, 3,4, 1,3, *2,5, *1,6, *1,6
1Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin and German Centre for Cardiovascular Research (DZHK), 2Department of Experimental and Clinical Medicine, University of Florence, 3Berlin Institute of Health, 4Department of Cardiology, German Heart Centre Berlin (DHZB), 5Sod of Interdisciplinary Internal Medicine, Azienda Ospedaliera Universitaria Careggi (AOUC), 6Department of General and Interventional Cardiology, Helios Klinikum Erfurt
* These authors contributed equally

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In deze studie beschrijven we het proces van T-lymfocytenisolatie uit verse monsters van verkalkte aortakleppen en de analytische stappen van T-celklonen voor de karakterisering van de adaptieve leukocytensubsets met behulp van flowcytometrie-analyse.

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Immunologie en infectie Aortaklepziekte aortastenose T-cellenextractie TAVI buffy coat flowcytometrie-analyse adaptieve immuniteit.
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