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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualização de SARS-CoV-2 usando Imuno RNA-Fluorescence In Situ Hibridização
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Visualização de SARS-CoV-2 usando Imuno RNA-Fluorescence In Situ Hibridização

Article DOI: 10.3791/62067-v • 05:23 min • December 23rd, 2020
December 23rd, 2020 •
1, 2, 1, 1,2, 1,2, 3,4, 3,4, 1
1Malopolska Centre of Biotechnology, Jagiellonian University, 2Microbiology Department Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 3Department of Cardiac, Vascular and Endovascular Surgery and Transplantology, The Medical University of Silesia in Katowice, 4Silesian Centre for Heart Diseases

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Aqui, descrevemos um método simples que combina fluorescência de RNA na hibridização situ (RNA-FISH) com imunofluorescência para visualizar síndrome respiratória aguda grave coronavírus 2 (SARS-CoV-2) RNA. Este protocolo pode aumentar a compreensão das características moleculares das interações sars-cov-2 RNA-host em um nível de célula única.

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Imunologia e Infecção Problema 166 SARS-CoV-2 RNA-FISH Reação em cadeia de Hibridização Imunofluorescência Epitélio das Vias Aéreas Humanas (HAE) Permeabilização Microscopia Confocal
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