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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualisering av SARS-CoV-2 med immun RNA-fluorescens i Situ hybridisering
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Visualisering av SARS-CoV-2 med immun RNA-fluorescens i Situ hybridisering

Article DOI: 10.3791/62067-v • 05:23 min • December 23rd, 2020
December 23rd, 2020 •
1, 2, 1, 1,2, 1,2, 3,4, 3,4, 1
1Malopolska Centre of Biotechnology, Jagiellonian University, 2Microbiology Department Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 3Department of Cardiac, Vascular and Endovascular Surgery and Transplantology, The Medical University of Silesia in Katowice, 4Silesian Centre for Heart Diseases

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Här beskriver vi en enkel metod som kombinerar RNA fluorescens in situ hybridisering (RNA-FISH) med immunofluorescens för att visualisera allvarliga akut respiratoriska syndrom coronavirus 2 (SARS-CoV-2) RNA. Detta protokoll kan öka förståelsen för de molekylära egenskaperna hos SARS-CoV-2 RNA-värd interaktioner på en cell nivå.

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Immunologi och infektion Utgåva 166 SARS-CoV-2 RNA-FISH Hybridiseringskedja reaktion Immunofluorescence Human Airway Epitel (HAE) Permeabilization Confocal mikroskopi
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