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JoVE Journal Bioengineering
DNA-gebonden RNA-polymerase voor programmeerbare in vitro transcriptie en moleculaire berekening
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DNA-gebonden RNA-polymerase voor programmeerbare in vitro transcriptie en moleculaire berekening

Article DOI: 10.3791/62073-v • 09:26 min • December 29th, 2021
December 29th, 2021 •
1, 1, 1
1Institute of Biomedical Engineering, University of Toronto

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We beschrijven de engineering van een nieuw DNA-gebonden T7-RNA-polymerase om in vitro transcriptiereacties te reguleren. We bespreken de stappen voor eiwitsynthese en karakterisering, valideren proof-of-concept transcriptionele regulatie en bespreken de toepassingen ervan in moleculaire computing, diagnostiek en moleculaire informatieverwerking.

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Bio-engineering Nummer 178 dynamische DNA-nanotechnologie moleculaire programmering moleculaire computing in vitro gencircuit in vitro transcriptie teen-gemedieerde strengverplaatsing
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