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JoVE Journal Neuroscience
Polisome Profilleme Kullanarak Gelişen Fare Beyninde Çeviri Analizi
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Polisome Profilleme Kullanarak Gelişen Fare Beyninde Çeviri Analizi

Article DOI: 10.3791/62088-v • 08:38 min • May 22nd, 2021
May 22nd, 2021 •
1,3, 2,3, 1,2,3
1Department of Biochemistry and Molecular Biology; Cumming School of Medicine, University of Calgary, 2Department of Medical Genetics, Cumming School of Medicine, University of Calgary, 3Alberta Children's Hospital Research Institute, University of Calgary

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Memeli beyninin gelişimi, gen ekspresyonunun çeviri düzeyinde uygun şekilde kontrolini gerektirir. Burada, gelişmekte olan beyindeki mRNA'ların çevirisel durumunu değerlendirmek için montajı kolay bir sakkaroz gradyan ve fraksiyonasyon platformuna sahip bir polisome profilleme sistemini tarif ediyoruz.

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