Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Optimized Incorporation of Alkynyl Fatty Acid…
JoVE Journal
Biochemistry

This content is Open Access.

JoVE Journal Biochemistry
Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry
Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay
Journal (Biochemistry)
Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay
Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo
Journal (Biochemistry)
Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo
 

Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry

Article DOI: 10.3791/62107-v • 07:27 min • April 9th, 2021
April 9th, 2021 •
1, 1, 1
1Department of Biology, University of Waterloo

Chapters

Summary
Automatic Translation

The study of fatty acylation has important implications in cellular protein interactions and diseases. Presented here is a modified protocol to improve click chemistry detection of fatty acylated proteins, which can be applied in various cell types and combined with other assays, including pulse-chase and mass spectrometry.

Tags

Alkynyl Fatty Acid Analogs Click Chemistry Fatty Acylated Proteins Delivery Of Fatty Acids Labeling Protocol Labeling Media HEK-293 T-cells Metabolic Labeling Saponify Fatty Acid Analogs Potassium Hydroxide
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter