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Journal / Biochemistry / Site Specifik lysin acetylering af Histoner for…
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Biochemistry

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JoVE Journal Biochemistry
Site Specifik lysin acetylering af Histoner for nukleosom rekonstitution ved hjælp af genetisk kode ekspansion i Escherichia coli
Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
Journal (Biochemistry)
Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag
Journal (Biochemistry)
Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag
 
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Site Specifik lysin acetylering af Histoner for nukleosom rekonstitution ved hjælp af genetisk kode ekspansion i Escherichia coli

Article DOI: 10.3791/62113-v • 07:26 min • December 26th, 2020
December 26th, 2020 •
1, 1
1Department of Chemistry, Texas A&M University

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Her præsenterer vi en metode til at udtrykke acetylerede histoneproteiner ved hjælp af genetisk kodeudvidelse og samle rekonstituerede nukleosomer in vitro.

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Biokemi Problem 166 nukleosom acetylering pyrrolysin genetisk kodeudvidelse postoversættelsesændringer proteinmodifikationer histoner
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