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Wide-Field, Real-Time Imaging af lokale og systemiske sårsignaler i Arabidopsis

Article DOI: 10.3791/62114-v • 06:50 min • June 4th, 2021
June 4th, 2021 •
1, 1, 1, 2, 1,2
1Department of Biochemistry and Molecular Biology, Saitama University, 2Department of Botany, University of Wisconsin

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Ekstracellulær glutamat-udløst systemisk calcium signalering er afgørende for induktion af planteforsvar svar på mekaniske sår og planteædende angreb i planter. Denne artikel beskriver en metode til at visualisere den rumlige og tidsmæssige dynamik i begge disse faktorer ved hjælp af Arabidopsis thaliana planter udtrykke calcium- og glutamat-følsomme fluorescerende biosensorer.

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Biologi Problem 172 calcium GCaMP3 genetisk kodede fluorescerende biosensorer glutamat iGluSnFR langdistancesignal sårende
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