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JoVE Journal Neuroscience
En platebasert analyse for måling av endogen monoaminfrigjøring i akutte hjerneskiver
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En platebasert analyse for måling av endogen monoaminfrigjøring i akutte hjerneskiver

Article DOI: 10.3791/62127-v • 07:56 min • August 11th, 2021
August 11th, 2021 •
*1, *2, 3, 2
1Department of Medicine, School of Medicine, Universidad de Atacama, 2Department of Molecular, Cellular, and Biomedical Sciences, City University of New York School of Medicine at City College, 3Department of Pharmacology and Therapeutics, University of Florida College of Medicine
* These authors contributed equally

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Denne metoden introduserer en enkel teknikk for påvisning av endogen monoaminfrigjøring ved hjelp av akutte hjerneskiver. Oppsettet bruker en 48-brønns plate som inneholder en vevsholder for monoaminfrigjøring. Frigjort monoamin analyseres av HPLC kombinert med elektrokjemisk deteksjon. I tillegg gir denne teknikken en screeningmetode for narkotikaoppdagelse.

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Nevrovitenskap utgave 174 monoaminfrigjøring akutte hjerneskiver monoaminer dopamin noradrenalin serotonin nevrotransmisjon
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