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Sarcomere Shortening of Pluripotent Stem Cell-Derived Cardiomyocytes using Fluorescent-Tagged Sarcomere Proteins.
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Sarcomere Shortening of Pluripotent Stem Cell-Derived Cardiomyocytes using Fluorescent-Tagged Sarcomere Proteins.

Article DOI: 10.3791/62129-v • 08:37 min • March 3rd, 2021
March 3rd, 2021 •
*1, *1, 1,2, 3,4, 4, 4, 1, 1
1Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, 2Department of Pediatrics, Jichi Medical University, 3Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, 4Department of Precision Mechanics, Faculty of Science and Engineering, Chuo University
* These authors contributed equally

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Summary
Automatic Translation

This method can be used to examine sarcomere shortening using pluripotent stem cell-derived cardiomyocytes with fluorescent-tagged sarcomere proteins.

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Cardiomyocytes Fluorescent-tagged Sarcomere Proteins Sarcomere Function In Vitro AAV-based Transduction Induced Pluripotent Stem Cells Cardiomyopathy Therapy Disease-related Sarcomere Dysfunction Cardiology Field Pediatric Cardiology Study Skeletal Muscle Dysfunction Myopathy Differentiation Induction Laminin-511 E8 Recombinant Trypsin FBS AK02N Medium
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