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JoVE Journal Genetics
Brug af GAL4-UAS System for Funktionel Genetik i Anopheles gambiae
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Brug af GAL4-UAS System for Funktionel Genetik i Anopheles gambiae

Article DOI: 10.3791/62131-v • 06:29 min • April 15th, 2021
April 15th, 2021 •
1, 1, 1,2,3, 1, 1, 1, 1
1Department of Vector Biology, Liverpool School of Tropical Medicine, 2IMBB FORTH, 3Department of Biology, University of Crete

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Det tværpolitiske GAL4-UAS-system er et alsidigt værktøj til modifikation af genekspression på en kontrolleret spatiotemporal måde, som muliggør funktionel genetisk analyse i Anopheles gambiae. De procedurer, der er beskrevet for brug af dette system, er en semi-standardiseret kloningsstrategi, sexing og screening af pupper til fluorescerende proteinmarkører og embryofiksering.

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Genetik Udgave 170 3xP3 Lokalisering Endogenous Expression RNAi Knockdown Mikroskopi tværpolitisk Insekticidresistens vektorkapacitet.
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