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JoVE Journal
Biology

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哺乳动物原色素上皮细胞的分离、培养和基因工程用于非病毒基因治疗
 
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哺乳动物原色素上皮细胞的分离、培养和基因工程用于非病毒基因治疗

Article DOI: 10.3791/62145-v 09:46 min February 26th, 2021
February 26th, 2021

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

在这里,提出了一种从各种哺乳动物(小鼠,大鼠,兔子,猪和牛)中分离和转染原代虹膜和视网膜色素上皮细胞的方案。该方法非常适合在各种设置中研究眼部基因治疗方法,以进行离体分析和可转移到人类的体内研究。

Tags

生物学,第168期,眼部基因治疗,视网膜变性,RPE细胞,IPE细胞,细胞分离, 睡美人 转座子,非病毒基因治疗,PEDF,GM-CSF
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