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Journal / Neuroscience / 4-dimensionel billeddannelse af zebrafisk optic…
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Neuroscience

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JoVE Journal Neuroscience
4-dimensionel billeddannelse af zebrafisk optic Cup morfogenese
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4-dimensionel billeddannelse af zebrafisk optic Cup morfogenese

Article DOI: 10.3791/62155-v • 07:26 min • May 26th, 2021
May 26th, 2021 •
1, 1, 1
1Department of Human Genetics, University of Utah

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Denne protokol beskriver en tilgang til toto mærkning og flerdimensional billeddannelse af zebrafisk tidlig øjenudvikling. Vi beskriver mærkning, indlejring, og firedimensionelle (4D) billedbehandling ved hjælp af laserscanning confocal mikroskopi, og overvejelser for at optimere erhvervelsen af datasæt til dissekering mekanismer optik kop morfogenese.

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Neurovidenskab billeddannelse mikroskopi timelapse morfogenese optisk vesikel optisk kop udvikling zebrafisk øje nethinden
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