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Biology

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Generation af Murine Primary Colon Epitel Monolayers fra Tarmkrypter
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Generation af Murine Primary Colon Epitel Monolayers fra Tarmkrypter

Article DOI: 10.3791/62156-v • 06:57 min • February 6th, 2021
February 6th, 2021 •
1, 1, 1, 1, 1
1Department of Pathology, School of Medicine, University of Michigan

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I denne protokol beskriver vi, hvordan man genererer murin primære epitel kolonmonomer direkte fra tarmkrypter. Vi tilbyder eksperimentelle tilgange til at generere sammenflydende monolayers på gennemtrængelige filtre, sammenflydende monolayers til ridseredning og biokemiske undersøgelser og sparsomme og sammenflydende monolayers til immunfluorescensanalyse.

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Biologi Problem 168 primær kultur 2D-enteroider 2D-kolonoider epitelceller epitelmonomer tarmkrypter
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