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JoVE Journal
Biology

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来自肠道密码的穆林原结肠上皮单层的生成
 
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来自肠道密码的穆林原结肠上皮单层的生成

Article DOI: 10.3791/62156-v 06:57 min February 6th, 2021
February 6th, 2021

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

在此协议中,我们描述了如何直接从肠道密码生成穆林原发性上皮结肠单层。我们提供实验方法,在透气过滤器上生成汇流单层,在划痕伤口愈合和生化研究中产生汇流单层,以及用于免疫荧光分析的稀疏和共生单层。

Tags

生物学,第168期,初级培养,2D肠类,2D结肠类,上皮细胞,上皮单层,肠道密码
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